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All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may. Permission can be requested from either ISO at the address. Annex A normative Flow diagram of the procedure Annex B normative Culture media and reagents Annex C informative Results of the interlaboratory study ISO the International Organization for Standardization is a worldwide federation of national standards.
The work of preparing International Standards is normally carried out. In particular the different approval criteria needed for the. This document was drafted in accordance with the. Attention is drawn to the possibility that some of the elements of this document may be the subject of. ISO shall not be held responsible for identifying any or all such patent rights. Details of. Any trade name used in this document is information given for the convenience of users and does not.
This second edition cancels and replaces the first edition ISO , which has been technically. In this case, different methods, which are specific to.
Nevertheless, every. When this part of ISO is next reviewed, account will be taken of all information then available. It is hoped that when such standards are reviewed they will be changed to comply.
It is mainly concerned with Staphylococcus aureus, but also with S. Nevertheless, it is recommended to use the. These latter strains may be confused with other bacteria but they. ISO , such as the sensitivity to lysostaphin, the production of haemolysin, thermostable nuclease. The main technical changes listed in the Foreword, introduced in this document compared to ISO This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci.
The following documents are referred to in the text in such a way that some or all of their content. ISO all parts , Microbiology of the food chain — Rules for the preparation of the test sample, of initial. ISO and IEC maintain terminological databases for use in standardization at the following addresses:. Baird-Parker agar medium and which show a positive coagulase reaction in a tube test or on rabbit. Inoculation of the surface of a solid selective culture medium, with a specified quantity of the test.
Inoculation, under the same conditions, using decimal dilutions of the test sample or of the initial. Follow current laboratory practices in accordance with ISO The composition of culture media. For performance testing of culture media,. Disposable equipment is an acceptable alternative to reusable glassware if it has suitable specifications.
Bottles or flasks with non-toxic. Sterile Pasteur or graduated pipettes and pipettor tips should be fitted with a non-absorbent cotton. The pH meter shall be equipped with either manual or. Standard dealing with the product concerned. If there is no specific International Standard dealing. It is important that the laboratory receive a sample which is truly representative and has not been.
Standard dealing with the product concerned: follow the procedures specified in ISO all parts. If there is no specific International Standard, it is recommended that the. For enumeration. Repeat the procedure for the 10 dilution and for further decimal dilutions if necessary. If only. Also prepare duplicate using an additional three small agar. Allow the plates to dry with their lids on for. For the enumeration, only retain plates containing a maximum of colonies, including a maximum of.
Select for confirmation 9. NOTE 1 Typical colonies are black or grey, shining and convex 1 mm to 1,5 mm in diameter after incubation. After incubation for at least 24 h, an opalescent ring immediately in contact.
NOTE 2 Atypical colonies have the same size as typical colonies and can present one of the following.
Atypical colonies are formed mainly by strains of coagulase-positive staphylococci contaminating,. They are less often formed by strains of coagulase-. NOTE 3 Other colonies are all the remaining colonies possibly present on the plates that do not show the. NOTE 4 Bacteria belonging to genera other than staphylococci may give colonies with an appearance similar. Microscopic examination of Gram stain, before confirmation, will enable the distinction of other.
Select all such colonies for confirmation within the. The confirmation of coagulase-positive staphylococci is undertaken by a tube test 9. From the surface of each selected colony 9. Aseptically add 0,1 ml of each culture to 0,3 ml of the rabbit plasma B.
By tilting the tube, examine for clotting of the plasma after 4 h to 6 h of incubation and, if the test is. As a negative control, for each batch of plasma, add 0,1 ml of sterile brain-heart infusion broth B. This document specifies the general principle and the technical protocol for the validation of alternative confirmation methods for microbiology in the food chain.
This document compares the result of the alternative confirmation method against the confirmation procedure of a reference method or, if needed, a reference confirmation method e. This document is applicable to the validation of alternative confirmation methods used for the analysis detection or quantifi This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty MU associated with quantitative results in microbiology of the food chain.
It is applicable to the quantitative analysis of: — products intended for human consumption or the feeding of animals; — environmental samples in the area of food production and food handling; — samples at the stage of primary production. The quantitative analysis is typically carried out by enumerat This method is not validated for detection of the target viruses in other foodstuffs including multi-component foodstuffs , or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or o This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.
The use of this document can be extended to yeasts that do not form mycelium. This document specifies requirements and gives guidelines for the organization of proficiency testing PT schemes for microbiological examinations of a foods and beverages, b feeding animals, c environmental samples from food and feed production and handling, and d primary production stages. This document is also applicable to the microbiological examination of water where water is either used in food production or is regarded as a food in national legislation.
This document relates t This document specifies horizontal methods for sampling techniques using contact plates, stick swabs, sponges and cloths on surfaces in the food chain environment in order to detect and enumerate culturable microorganisms such as pathogenic or non-pathogenic bacteria or yeasts and moulds.
NOTE The term "environment" means any item in contact with the food product or likely to represent a contamination or recontamination source; for example, material, premises or operators.
This document does n Your shopping cart is empty! Back Technical Committee. Drafting Committee. Current Stage. Completion Date. Ref Project. EN ISO - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci Staphylococcus aureus and other species - Part 1: Technique using Baird-Parker agar medium ISO Buy Standard.
Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission.
The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee.
International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. In particular the different approval criteria needed for the different types of ISO documents should be noted.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. This second edition cancels and replaces the first edition ISO , which has been technically revised. In this case, different methods, which are specific to these products, may be used if absolutely necessary for justified technical reasons.
Nevertheless, every attempt should be made to apply this horizontal method as far as possible. When this part of ISO is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed and the reasons for deviations from this method in the case of particular products.
prEN ISO 6888-1
ISO 6888-1:1999/Amd 2:2018